منابع مشابه
Structure and function of urea amidolyase
Urea is the degradation product of a wide range of nitrogen containing bio-molecules. Urea amidolyase (UA) catalyzes the conversion of urea to ammonium, the essential first step in utilizing urea as a nitrogen source. It is widely distributed in fungi, bacteria and other microorganisms, and plays an important role in nitrogen recycling in the biosphere. UA is composed of urea carboxylase (UC) a...
متن کاملPurification and properties of the urea amidolyase from Candida utilis.
Urea amidolyase was purified to homogeneity from extracts of Candida utilis. The purification involves protamine sulfate precipitation, ammonium sulfate precipitation, polyethylene glycol precipitation, Sepharose 6B gel filtration, DEAE-cellulose column chromatography, and hydroxylapatite column chromatography. The final preparation is pure as judged by disc-gel electrophoresis. The molecular w...
متن کاملUrea amidolyase. I. Properties of the enzyme from Candida utilis.
Candida utilis contains an inducible enzyme, urea amidolyase, which catalyzes the decomposition of urea with formation of carbon dioxide and ammonia. This reaction is accompanied by the cleavage of ATP with liberation of equimolar amounts of ADP and inorganic phosphate, is strictly dependent for activity on divalent (Mg++ or Mn++) and monovalent (K+, Rb+, Csf, or NH4+) cations, and requires cat...
متن کاملUrea amidolyase of Candida utilis. Characterization of the urea cleavage reactions.
Evidence is presented that the enzymes catalyzing the three reactions involved in urea cleavage in Candida utilis, biotin carboxylation, urea carboxylation, and allophanate hydrolysis occur as a complex of enzymes. The allophanate-hydrolyzing activity could not be separated from the urea-cleaving activity using common methods of protein purification. Further, urea cleavage and allophanate hydro...
متن کاملSeparation and differential sensitivity toward avidin of carbamyl phosphate synthetase and urea amidolyase.
This enzyme is remarkably sensitive to inhibition by highly purified egg white avidin, a phenomenon which can be completely prevented by inclusion of excess biotin in the assay system. Inasmuch as avidin has been found to selectively inhibit all known biotinenzymes and, indeed, is now accepted as a diagnostic tool for the detection of such reactions [2], the proposal was made that UALase belong...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44559-6